The Compact Culture System makes bench-top-scale culture of zooplankton possible. Aeration and waste control are integrated into the design using a 20-L bucket. This Compact Culture System will allow you to produce millions of rotifers per day, perfectly enriched for larval fish and invertebrates.
” The saltwater rotifer, Brachionus plicatilis, is widely used in the aquaculture industry as a prey item for first-feeding fishes due to its ease of culture, small size, rapid reproductive rate, and amenability to enrichment with nutrients. Despite the distinct advantages of this approach, rotifers have only been sporadically utilized for rearing larval zebrafish, primarily because of the common misconception that maintaining cultures of rotifers is difficult and excessively time-consuming. Here we present simple methods for maintaining continuous cultures of rotifers capable of supporting even the very largest zebrafish aquaculture facility, with minimal investments in materials, time, labor, and space. Examples of the methods’ application in one large, existing facility is provided, and troubleshooting of common problems is discussed “
From Lawrence, C., Best, J., Cockington, J., Henry, E. C., Hurley, S., James, A., et al. The Complete and Updated “Rotifer Polyculture Method” for Rearing First Feeding Zebrafish. J. Vis. Exp. (107), e53629, doi:10.3791/53629 (2016).